Alaabta
Nooca-hoosaadka H7 Hargabka Avian-ka Antibody ELISA Qalabka Tijaabada
Nooca-hoosaadka H7 Hargabka Avian-ka Antibody ELISA Qalabka Tijaabada
| Soo koobid | Qalabka baaritaanka AIV-H7 ee ka hortagga unugyada ELISA ayaa loo istcmaalaa in lagu ogaado H7-hoosaadka hargabka Avian-ka difaaca jirka ee serum |
| Mabda'a | Qalabka baaritaanka AIV-H7 ee ELISA wuxuu u istcmaalaa ogaanshaha H7 Subtype-ka-hortagga hargabka Avian-ka ee serumka, si loola socdo antibody ka dib difaaca AIV-H7 iyo ogaanshaha serological ee caabuqa ee Avian |
| Bartilmaameedyada ogaanshaha | AIV-H7 antibody |
| Tusaale | Serum
|
| Tirada | 1 xirmo = 192 Imtixaan |
|
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
|
Xog
Hargabka avian, oo si aan rasmi ahayn loo yaqaan ifilada avian ama hargab shimbireedka, waa hargab kala duwan oo uu keeno fayras la qabsadayshimbiraha.
Nooca halista ugu badan leh waa hargabka avian-ka (HPAI).Hargabka shimbiruhu wuxuu la mid yahayifilada doofaarka, ifilada eyda, hargabka faraska iyo
Hargabka bini'aadamka waa cudur ay sababaan noocyada fayrasyada hargabka kuwaas oo la qabsaday qof gaar ah.
Saddexda nooc ee fayraska hargabka (A,BiyoC), fayraska hargabka A waa azoonoticinfekshanka kaydka dabiiciga ah ku dhawaad
Hargabka avian-ka, ujeeddooyinka intooda badan, waxa loola jeedaa fayraska hargabka A.
Mabda'a Imtixaanka
Xirmadani waxay isticmaashaa habka ELISA tartanka si ay hore ugu dahaadhay antigens AIV-H7 ceelasha microplate.Marka la tijaabinayo, ku dar muunadda serum la badhxay iyo ensaymes oo lagu calaamadeeyay anti-AIV-H7 monoclonal antibody, ka dib marka la isku duro, haddii ay jiraan antibody AIV-H7, waxay ku dari doontaa antigen-ka hore loo dahaadhay, antibody muunada muunad ayaa xannibaya isku dhafka monoclonal antibody iyo ka hor. antigen-dahaarka leh;iska tuur isku dhafka enzyme-ka ee aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
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