Alaabta
Xirmada ELISA ee Hargabka-Avian-ka
Xirmada ELISA ee Hargabka-Avian-ka
| Soo koobid | Loo isticmaalo in lagu ogaado unugyada gaarka ah ee ka hortagga fayraska hargabka avian (AIV) ee serumka |
| Mabda'a | Xirmada Elisa ee ka hortagga hargabka Avian waxaa loo isticmaalaa in lagu ogaado antibody gaar ah oo ka hortagaya fayraska hargabka Avian (AIV) ee serum, si loola socdo antibody ka dib difaaca AIV iyo ogaanshaha serological ee caabuqa ee Avian. |
| Bartilmaameedyada ogaanshaha | Hargabka Avian Antibody |
| Tusaale | Serum
|
| Tirada | 1 xirmo = 192 Imtixaan |
|
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
|
Xog
Hargabka avian, oo si aan rasmi ahayn loo yaqaan ifilada avian ama hargab shimbireedka, waa hargab kala duwan oo uu keeno fayras la qabsadayshimbiraha.
Nooca halista ugu badan leh waa hargabka avian-ka (HPAI).Hargabka shimbiruhu wuxuu la mid yahayifilada doofaarka, hargab eyda, faras
hargabka iyo hargabka bini'aadamka sida jirro ay keenaan noocyada fayraska hargabka kuwaas oo la qabsaday mid gaar ah
martigeliyaha.Saddexda nooc ee fayraska hargabka (A,BiyoC), fayraska hargabka A waa azoonoticcaabuqa dabiiciga ah
kaydka guud ahaan shimbiraha.Hargabka avian, ujeedooyinka badankooda, waxa loola jeedaa fayraska hargabka A.
Mabda'a Imtixaanka
Qalabkani waxa uu isticmaalaa habka ELISA block, AIV antigen waxa hore loogu dahaadhay microplate.Marka la tijaabinayo, ku dar muunada serum la badhxay, ka dib marka la isku daro, haddii ay jirto antibody gaar ah oo AIV ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee dhaqidda;ka dibna ku dar ensaymes calaamadeysan anti-AIV monoclonal antibody, antibody ee muunada xannibi isku darka monoclonal antibody iyo antigen pre-daahan;iska tuur isku xidhka ensaymeska aan la isku darin ee la dhaqo.Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
