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Ukunta Dhibta Syndrome1976 fayraska difaaca jirka ELISA Ki Sawirka la soo bandhigay
  • Ukunta hoos u dhacda 1976 fayraska difaaca jirka ELISA Ki
  • Ukunta hoos u dhacda 1976 fayraska difaaca jirka ELISA Ki

Ukunta hoos u dhacda 1976 fayraska difaaca jirka ELISA Ki

Koodhka badeecada:

Shayga Magaca: Eggs Drop Syndrome1976 fayraska ka hortagga unugyada ELISA

Soo koobid: Fayraska Eggs Drop Syndrome 1976 (EDS76) Qalabka Ab Elisa waxa loo isticmaalaa in lagu ogaado antibody gaar ah oo liddi ku ah EDS76 ee serum si tayo leh.Si loola socdo antibody ka dib EDS76 difaaca iyo baaritaanka serological ee caabuqa ee Avian.

Bartilmaameedyada Ogaanshaha: Eggs Drop Syndrome1976 fayraska Antibody

Tusaalaha Tijaabada: Serum

Tilmaam: 1 xirmo = 192 Imtixaan

Kaydinta: Dhammaan reage-yaasha waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

Waqtiga kaydinta: 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.


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Ukunta hoos u dhacda 1976 fayraska difaaca jirka ELISA Ki

Soo koobid   Use si loo ogaado antibody gaar ah oo liddi ku ah EDS76 ee serum si tayo leh.
Mabda'a

Qalabka loo yaqaan 'Eggs Drop Syndrome' 1976 fayraska (EDS76) Qalabka Ab Elisa waxa loo isticmaalaa in lagu ogaado antibody gaar ah oo liddi ku ah EDS76 oo serum tayo leh.Si loola socdo antibody ka dib EDS76 difaaca iyo baaritaanka serological ee caabuqa ee Avian.
Bartilmaameedyada ogaanshaha  Aantibody ka dhanka ah EDS76 ee serum
Tusaale Serum

 
Tirada 1 xirmo = 192 Imtixaan
 

 

Deganaanshaha iyo Kaydinta

1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 

 

 

Xog

Egg Drop Syndrome (EDS-76) waa cudur faafa oo ba'an oo uu keeno adenovirus group III ee Adenoviridae Avian Virus genus oo leh hemagglutination.Qaar ka mid ah beeraha digaaga, wax soo saarka ukunta tirada digaaga ayaa si lama filaan ah hoos ugu dhacay, waxaana la soo saaray ukumo qallafsan sida ukunta qolof jilicsan, ukun qolof yar, iyo ukun qolof dhuuban ayaa la soo saaray isku mar.Guud ahaan koorsada cudurku waxay socotaa 5-6 toddobaad, ka dib markaa heerka wax soo saarka ukunta ayaa si tartiib tartiib ah kor u kaca, laakiin way adagtahay in la gaaro heerka ka hor inta aan la dhicin.

Mabda'a Imtixaanka

Xirmadani waxay isticmaashaa habka ELISA aan tooska ahayn, EDS76 antigen oo saafi ah ayaa horay loogu dahaadhay fiilooyinka ceelka-ceelka-yar ee enzyme.,Haddii ay jiraan EDS76virus gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, ka hortagga unugyada aan la isku darin iyo qaybaha kale ee dhaqidda;ka dibna ku dar enzim lagu calaamadeeyay anti-EDS76 fayraska monoclonal antibody, ka dibna isku darka antibody monoclonal iyo antigen horay loo dahaadhay;iska tuur isku dhafka enzyme-ka ee aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay bixiso Enzyme catalysis waa saamiga lidka ku ah maadada antibody-ka ee muunada, isticmaal akhristaha ELISA 450nm mawjada si aad u cabbirto nuugista Qiimaha A ee ceelasha falcelinta ka dib marka lagu daro xalka joojinta
si loo joojiyo falcelinta.

Nuxurka

 

Reagent

Mugga

96 Tijaabooyin/192 Tijaabooyin
1
Microplate-dahaarka antigen

 

1ea/2e
2
 Xakamaynta taban

 

2.0ml
3
 Xakamaynta togan

 

1.6ml
4
 Tusaalaha dareeraha

 

100ml
5
Xalka dhaqidda (10X xoogga saaray)

 

100ml
6
 Enzyme conjugate

 

11/22 ml
7
 Substrate

 

11/22 ml
8
 Xalka joojinta

 

15ml
9
Xireeyaha saxan koollada

 

2ea/4e
10 mikroplate serum dilution

1ea/2e
11  Tilmaanta

1 pcs

 

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