Alaabta
Qalabka ELISA ee Cudurka Cudurka Newcastle
Qalabka ELISA ee Cudurka Cudurka Newcastle
| Soo koobid | Ogaanshaha Antibody-ga gaarka ah ee ka-hortagga cudurrada Newcastle |
| Mabda'a | Qalabka difaaca jirka ee cudurka Newcastle ee Elisa waxaa loo isticmaalaa in lagu ogaado antibody gaar ah oo ka dhan ah cudurka Newcastle Virus (NDV) ee serum, si loola socdo antibody ka dib difaaca NDViyo ogaanshaha serological ee caabuqa ee Avian.
|
| Bartilmaameedyada ogaanshaha | Cudurka Newcastle antibody |
| Tusaale | Serum
|
| Tirada | 1 xirmo = 192 Imtixaan |
|
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Xog
Cudurka Newcastle waa cudur fayras ah oo la kala qaado oo saameeya shimbiraha gudaha iyo kuwa duurjoogta ah oo badan;way u gudbi kartaa dadkamarar dhif ah waxay u keeni kartaa xummad fudud iyo calaamado hargab u eg iyo/ama indho-sarcaadka dadka.Saameynteeda ayaa ah mid aad looga xuso digaaga gudaha sababtoo ah nuglaanshahooda sare iyo suurtagalnimada saameynta xun ee epizootic ee warshadaha digaaga.Wadamo badan buu ku faafaa.Lama garanayo wax daawayn ah oo loogu talagalay, laakiin isticmaalka tallaalada kahortagga ah iyo tallaabooyinka fayadhowrka ayaa yareynaya suurtagalnimada in dillaaco.
Mabda'a Imtixaanka
Qalabkani waxa uu isticmaalaa habka ELISA block, NDV antigen waxa hore loogu dahaadhay microplate.Marka la tijaabinayo, ku dar muunada serum la badhxay, ka dib marka la isku duro, haddii ay jirto antibody gaar ah NDV, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee dhaqidda;ka dibna ku dar ensaymes calaamadeysan anti-NDV monoclonal antibody, antibody muunada ku jira xanniba isku darka monoclonal antibody iyo antigen horay loo dahaadhay;iska tuur isku xidhka ensaymeska aan la isku darin ee la dhaqo. Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
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