Nooca Cudurka Lugaha iyo Afka O Ab ELISA Qalabka Imtixaanka Sawirka

Nooca Cudurka Cagaha iyo Afka O Qalabka Imtixaanka Ab ELISA

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Nooca Cudurka Cagaha iyo Afka O Qalabka Imtixaanka Ab ELISA

Soo koobid	Ogaanshaha gaar ahLug iyo Af antibodyNooca O
Mabda'a	Qalabka FMD Nooca Asia I ee ka hortagga unugga ELISA ee loo isticmaalo ogaanshaha unugyada ka-hortagga fayraska cudurrada cagta-iyo-afka ee serum ee doofaarrada, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD.
Bartilmaameedyada ogaanshaha	Lug iyo Af antibodyNooca O
Tusaale	Serum
Tirada	1 xirmo = 192 Imtixaan
Deganaanshaha iyo Kaydinta	1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

Xog

Cudurka cagaha iyo afka(FMDV) waa kancudur-sidahataasi waxay keentaacagaha iyo afka cudur.^[1]Waa apicornavirus, xubinta prototypical ee genusAphthovirus.Cudurka, kaas oo sababa finan (finan) afka iyo cagahalo'daDoofaarka, idaha, riyaha, iyo kuwa kaleqoob-ka-baxsanxooluhu aad bay u faafaan waana cudur daacuun weyn ku hayadhaqashada xoolaha

Mabda'a Imtixaanka

Habkani si toos ah ELISA,FMDVantigenis horay loogu dahaadhay fiilooyinka ceelka-yar ee enzyme.Marka la tijaabinayo, muunada serum ee dheeriga ah, ka dib marka la isku tallaalo, haddii uu jiro fayraska FMD antibody gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee dhaqidda;ka dibna ku dar konjugate enzyme, iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo.Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waa saamiga tooska ah ee ka-hortagga unugyada ee muunada.