Alaabta
Ukunta hoos u dhacda 1976 fayraska difaaca jirka ELISA Ki
Ukunta hoos u dhacda 1976 fayraska difaaca jirka ELISA Ki
| Soo koobid | Usi aad u ogaato antibody gaar ah oo liddi ku ah EDS76 ee serum si tayo leh. |
| Mabda'a | Qalabka loo yaqaan 'Eggs Drop Syndrome' 1976 fayraska (EDS76) Qalabka Ab Elisa waxa loo isticmaalaa in lagu ogaado antibody gaar ah oo liddi ku ah EDS76 oo serum tayo leh.Si loola socdo antibody ka dib EDS76 difaaca iyo baaritaanka serological ee caabuqa ee Avian. |
| Bartilmaameedyada ogaanshaha | Aantibody ka dhanka ah EDS76 ee serum |
| Tusaale | Serum
|
| Tirada | 1 xirmo = 192 Imtixaan |
|
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Xog
Egg Drop Syndrome (EDS-76) waa cudur faafa oo ba'an oo uu keeno adenovirus group III ee Adenoviridae Avian Virus genus oo leh hemagglutination.Beeraha digaagga qaarkood, wax-soo-saarka ukunta digaagga ee digaagga ayaa si lama filaan ah hoos ugu dhacay, waxaana la soo saaray ukumo qallafsan sida ukunta qolof-jilicsan, ukun qolof-yar, iyo ukun qolof-dhuuban ah ayaa la soo saaray isku mar.Guud ahaan koorsada cudurku waxay socotaa 5-6 toddobaad, ka dib markaa heerka wax soo saarka ukunta ayaa si tartiib tartiib ah kor u kaca, laakiin way adagtahay in la gaaro heerka ka hor inta aan hoos u dhicin.
Mabda'a Imtixaanka
Xirmadani waxay isticmaashaa habka ELISA aan tooska ahayn, EDS76 antigen-ka saafiga ah ayaa horay loogu dahaadhay fiilooyinka ceelka-ceelka-yar ee enzyme.,Haddii ay jiraan EDS76virus gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, ka hortagga unugyada aan la isku darin iyo qaybaha kale ee lagu dhaqo;ka dibna ku dar enzim lagu calaamadiyay anti-EDS76 fayraska monoclonal antibody, ka dibna isku darka antibody monoclonal iyo antigen horay loo dahaadhay;iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamadda buluuga ah ee Enzyme catalysis waxay u dhigantaa saamiga lidka ku ah maadada antibody-ka ee muunada, isticmaal akhristaha ELISA 450nm mawjada si aad u cabbirto nuugista Qiimaha A ee ceelasha falcelinta ka dib markaad ku darto xalka joojinta
si loo joojiyo falcelinta.
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
