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Hargabka Qalab ka-hortagga Jirka ELISA

Koodhka badeecada:

 

Shayga Magaca: Hargabka Qalabka ELISA ee ka-hortagga

Soo koobid: Hargabka Qalabka difaaca jirka ee Elisa waxa loo isticmaalaa in lagu ogaado antibody gaar ah oo lid ku ah Fayraska Hargabka A (Flu A) ee serum, si loola socdo antibody ka dib hargabka A iyo ogaanshaha serological ee caabuqa Avian, doofaarka iyo Equus.

Bartilmaameedyada ogaanshaha: Hargabka A antibody

Tusaalaha Tijaabada: Serum

Tilmaam: 1 xirmo = 192 Imtixaan

Kaydinta: Dhammaan reage-yaasha waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

Waqtiga kaydinta: 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 

 

 


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Hargabka Qalab ka-hortagga Jirka ELISA

Soo koobid Ogaanshaha Antibody-ka gaarka ah ee Hargabka Baaritaanka difaaca iyo serological ee caabuqa ee Avian, doofaarka iyo Equus.
Mabda'a

Hargabka Qalabka difaaca jirka ee Elisa waxa loo isticmaalaa in lagu ogaado antibody gaar ah oo lid ku ah IFayraska hargabka A (Flu A) ee serum, si loola socdo antibody ka dib hargabka difaaciyo ogaanshaha serological ee caabuqa ee Avian, doofaarka iyo Equus.

 

Bartilmaameedyada ogaanshaha  Hargabka A antibody
Tusaale Serum

 

Tirada 1 xirmo = 192 Imtixaan
 

 

Deganaanshaha iyo Kaydinta

1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 

 

 

Xog

Hargabka A waa cudur keenaha keena hargabka shimbiraha iyo naasleyda qaarkood.Waa fayras RNA ah, oo noocyo hoose ka mid ah oo laga soocay shimbiraha duurjoogta ah.Marmar, waxay ku faaftaa shimbiraha duurjoogta ah una gudubta digaagga, taasoo keeni karta cudurro daran, dillaaca, ama hargabka aadamiga.

Mabda'a Imtixaanka

Qalabkani waxa uu isticmaalaa habka ELISA ee xannibaya, FluA antigen waxa hore loogu dahaadhay microplate.Markaad tijaabinayso, ku dar muunada serum la badhxay, ka dib marka la isku tallaalo, haddii uu hargabku jiro Antibody gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee la dhaqo;dabadeed ku dar insaymka lagu calaamadeeyay hargabka Antibody-ka monoclonal, antibody muunada ku jira ayaa xannibaya isku darka antibody-ka monoclonal iyo antigen horay loo dahaadhay;iska tuur isku xidhka ensaymeska aan la isku darin ee la dhaqo. Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.

Nuxurka

 

Reagent

Mugga

96 Tijaabooyin/192 Tijaabooyin

1
Microplate-dahaarka antigen

 

1ea/2e

2
 Xakamaynta taban

 

2.0ml

3
 Xakamaynta togan

 

1.6ml

4
 Tusaalaha dareeraha

 

100ml

5
Xalka dhaqidda (10X xoogga saaray)

 

100ml

6
 Enzyme conjugate

 

11/22 ml

7
 Substrate

 

11/22 ml

8
 Xalka joojinta

 

15ml

9
Xireeyaha saxan koollada

 

2ea/4e

10 mikroplate serum dilution

1ea/2e

11  Tilmaanta

1 pcs

 


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