Soo koobid | Ogaanshaha Antibody-ka gaarka ah ee Hargabka Baaritaanka difaaca iyo serological ee caabuqa ee Avian, doofaarka iyo Equus. |
Mabda'a | Hargabka Qalabka difaaca jirka ee Elisa waxa loo isticmaalaa in lagu ogaado antibody gaar ah oo lid ku ah IFayraska hargabka A (Flu A) ee serum, si loola socdo antibody ka dib hargabka difaaciyo ogaanshaha serological ee caabuqa ee Avian, doofaarka iyo Equus.
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Bartilmaameedyada ogaanshaha | Hargabka A antibody |
Tusaale | Serum
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Tirada | 1 xirmo = 192 Imtixaan |
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Hargabka A waa cudur keenaha keena hargabka shimbiraha iyo naasleyda qaarkood.Waa fayras RNA ah, oo noocyo hoose ka mid ah oo laga soocay shimbiraha duurjoogta ah.Marmar, waxay ku faaftaa shimbiraha duurjoogta ah una gudubta digaagga, taasoo keeni karta cudurro daran, dillaaca, ama hargabka aadamiga.
Qalabkani waxa uu isticmaalaa habka ELISA ee xannibaya, FluA antigen waxa hore loogu dahaadhay microplate.Markaad tijaabinayso, ku dar muunada serum la badhxay, ka dib marka la isku tallaalo, haddii uu hargabku jiro Antibody gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee la dhaqo;dabadeed ku dar insaymka lagu calaamadeeyay hargabka Antibody-ka monoclonal, antibody muunada ku jira ayaa xannibaya isku darka antibody-ka monoclonal iyo antigen horay loo dahaadhay;iska tuur isku xidhka ensaymeska aan la isku darin ee la dhaqo. Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.
Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | mikroplate serum dilution | 1ea/2e | |
11 | Tilmaanta | 1 pcs |