Alaabta
Cudurka Cagaha iyo Afka Nooca A Qalabka Baaritaanka ELISA ee Ka-hortagga Jirka
Cudurka Cagaha iyo Afka Nooca A Qalabka Baaritaanka ELISA ee Ka-hortagga Jirka
| Soo koobid | Ogaanshaha gaar ahCudurka Cagaha iyo Afka Nooca A antibody |
| Mabda'a | Qalabka baaritaanka ELISA Nooca FMD-ga ah ee loo isticmaalo ogaanshaha unugyada ka-hortagga fayraska cudurada cagta-iyo-afka ee serum ee doofaarrada, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD. |
| Bartilmaameedyada ogaanshaha | Cudurka Cagaha iyo Afka Nooca A |
| Tusaale | Serum
|
| Tirada | 1 xirmo = 192 Imtixaan |
|
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Xog
Cudurka cagaha iyo afka(FMDV) waa kancudur-sidahataasi waxay keentaacagaha iyo afka cudur. Waa apicornavirus, xubinta prototypical ee genusAphthovirus.Cudurka, kaas oo sababa finan (finan) afka iyo cagahalo'daDoofaarka, idaha, riyaha, iyo kuwa kaleqoob-ka-baxsanxooluhu aad bay u faafaan waana cudur daacuun weyn ku hayadhaqashada xoolaha.
Mabda'a Imtixaanka
Xirmadani waxay isticmaashaa habka ELISA ee tartanka si ay hore ugu dahaadhay antigens-ka cudurka cagaha iyo afka ee ceelasha microplate.Marka la tijaabinayo, ku dar muunada serum la badhxay iyo ka-hortagga FMD Ab, ka dib marka la isku dhejiyo, haddii ay jiraan antibody FMD, waxay ku dari doontaa antigen-ka hore loo dahaadhay, antibody-ka muunadda ayaa xannibaya isku-darka anti-FMD antibody iyo antigen-ka hore loo daboolay;iska tuur isku dhafka enzyme-ka ee aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.
Serotypes
Cudurka cagaha iyo afkawaxay ku dhacdaa todobada waaweynserotypes: O, A, C, SAT-1, SAT-2, SAT-3, iyo Asia-1.Serotypes-yadani waxay muujinayaan qaar ka mid ah gobollada, iyo O serotype waa kuwa ugu badan.
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
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