Soo koobid | Ogaanshaha gaar ahLug iyo Af antibodyNooca O |
Mabda'a | Qalabka FMD Nooca Asia I ee ka hortagga unugga ELISA ee loo isticmaalo ogaanshaha unugyada ka-hortagga fayraska cudurrada cagta-iyo-afka ee serum ee doofaarrada, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD. |
Bartilmaameedyada ogaanshaha | Lug iyo Af antibodyNooca O |
Tusaale | Serum
|
Tirada | 1 xirmo = 192 Imtixaan |
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
|
Cudurka cagaha iyo afka(FMDV) waa kancudur-sidahataasi waxay keentaacagaha iyo afka cudur.[1]Waa apicornavirus, xubinta prototypical ee genusAphthovirus.Cudurka, kaas oo sababa finan (finan) afka iyo cagahalo'daDoofaarka, idaha, riyaha, iyo kuwa kaleqoob-ka-baxsanXayawaanku aad bay u faafaan waana cudur ba'andhaqashada xoolaha
Habkani si toos ah ELISA,FMDVantigenis horay loogu dahaadhay fiilooyinka ceelka-yar ee enzyme.Marka la tijaabinayo, muunada serum ee dheeriga ah, ka dib soo-baxa, haddii uu jiro fayraska FMD antibody gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee lagu dhaqo;ka dibna ku dar konjugate enzyme, iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo.Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waa saamiga tooska ah ee ka-hortagga unugyada ee muunada.
Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
1 |
| 1ea/2e | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | mikroplate serum dilution | 1ea/2e | |
11 | Tilmaanta | 1 pcs |