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Nooca Cudurka Cagaha iyo Afka O Qalabka Imtixaanka Ab ELISA

Koodhka badeecada:

Shayga Magaca: Nooca Cudurka Lugaha iyo Afka O Qalabka Tijaabada ELISA ee ka-hortagga

Soo koobid: Qalabka FMD Nooca O antibody ee ELISA oo loo isticmaalo in lagu ogaado unugyada ka-hortagga fayraska cagaha-iyo-afka ee ku jira serum-ka doofaarka, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD.

Bartilmaameedyada Ogaanshaha: Cudurka Cagaha iyo Afka Nooca O Antibody

Tusaalaha Tijaabada: Serum

Tilmaam: 1 xirmo = 192 Imtixaan

Kaydinta: Dhammaan reage-yaasha waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

Waqtiga kaydinta: 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.


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Nooca Cudurka Cagaha iyo Afka O Qalabka Imtixaanka Ab ELISA

Soo koobid Ogaanshaha gaar ahLug iyo Af antibodyNooca O
Mabda'a

Qalabka FMD Nooca Asia I ee ka hortagga unugga ELISA ee loo isticmaalo ogaanshaha unugyada ka-hortagga fayraska cudurrada cagta-iyo-afka ee serum ee doofaarrada, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD.

Bartilmaameedyada ogaanshaha Lug iyo Af antibodyNooca O
Tusaale Serum

 

Tirada 1 xirmo = 192 Imtixaan
 

 

Deganaanshaha iyo Kaydinta

1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 

 

 

Xog

Cudurka cagaha iyo afka(FMDV) waa kancudur-sidahataasi waxay keentaacagaha iyo afka cudur.[1]Waa apicornavirus, xubinta prototypical ee genusAphthovirus.Cudurka, kaas oo sababa finan (finan) afka iyo cagahalo'daDoofaarka, idaha, riyaha, iyo kuwa kaleqoob-ka-baxsanXayawaanku aad bay u faafaan waana cudur ba'andhaqashada xoolaha

Mabda'a Imtixaanka

Habkani si toos ah ELISA,FMDVantigenis horay loogu dahaadhay fiilooyinka ceelka-yar ee enzyme.Marka la tijaabinayo, muunada serum ee dheeriga ah, ka dib soo-baxa, haddii uu jiro fayraska FMD antibody gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee lagu dhaqo;ka dibna ku dar konjugate enzyme, iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo.Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waa saamiga tooska ah ee ka-hortagga unugyada ee muunada.

Nuxurka

 

Reagent

Mugga

96 Tijaabooyin/192 Tijaabooyin

1
Microplate-dahaarka antigen

 

1ea/2e

2
Xakamaynta taban

 

2ml

3
Xakamaynta togan

 

1.6ml

4
Tusaalaha dareeraha

 

100ml

5
Xalka dhaqidda (10X xoog leh)

 

100ml

6
Enzyme conjugate

 

11/22 ml

7
Substrate

 

11/22 ml

8
Xalka joojinta

 

15ml

9
Xireeyaha saxan koollada

 

2ea/4e

10 mikroplate serum dilution

1ea/2e

11 Tilmaanta

1 pcs

 


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