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Cudurka Cagaha iyo Afka Nooca A Qalabka Baaritaanka ELISA ee Ka-hortagga Jirka

Koodhka badeecada:

Shayga Magaca: Cudurka Lugaha iyo Afka Nooca A Qalabka Tijaabada ELISA ee Ka-hortagga

Soo koobid: Qalabka FMD-ga Nooca A ee ka hortaga ELISA ee loo isticmaalo in lagu ogaado unugyada ka-hortagga fayraska cagaha-iyo-afka ee ku jira serum-ka doofaarka, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD.

Bartilmaameedyada Ogaanshaha: Cudurka Cagaha iyo Afka Nooca A

Tusaalaha Tijaabada: Serum

Tilmaam: 1 xirmo = 192 Imtixaan

Kaydinta: Dhammaan reage-yaasha waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

Waqtiga kaydinta: 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 


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Cudurka Cagaha iyo Afka Nooca A Qalabka Baaritaanka ELISA ee Ka-hortagga Jirka

Soo koobid Ogaanshaha gaar ahCudurka Cagaha iyo Afka Nooca A antibody
Mabda'a

Qalabka baaritaanka ELISA Nooca FMD-ga ah ee loo isticmaalo ogaanshaha unugyada ka-hortagga fayraska cudurrada cagta-iyo-afka ee ku jira serumka doofaarrada, lo'da, idaha iyo riyaha si loo qiimeeyo difaaca tallaalka FMD.

Bartilmaameedyada ogaanshaha Cudurka Cagaha iyo Afka Nooca A
Tusaale Serum

 

Tirada 1 xirmo = 192 Imtixaan
 

 

Deganaanshaha iyo Kaydinta

1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 

 

 

Xog

Cudurka cagaha iyo afka(FMDV) waa kancudur-sidahataasi waxay keentaacagaha iyo afka cudur. Waa apicornavirus, xubinta prototypical ee genusAphthovirus.Cudurka, kaas oo sababa finan (finan) afka iyo cagahalo'daDoofaarka, idaha, riyaha, iyo kuwa kaleqoob-ka-baxsanXayawaanku aad bay u faafaan waana cudur ba'andhaqashada xoolaha.

Mabda'a Imtixaanka

Xirmadani waxay isticmaashaa habka ELISA ee tartanka si ay hore ugu dahaadhay antigens-ka cudurada cagaha iyo afka ee ceelasha microplate.Marka la tijaabinayo, ku dar muunadda serum la badhxay iyo ka-hortagga FMD Ab, ka dib marka la isku dhejiyo, haddii ay jirto antibody FMD, waxay ku dari doontaa antigen-ka hore loo dahaadhay, antibody-ka muunada ayaa xannibaya isku-darka anti-FMD antibody iyo antigen-ka hore loo dahaadhay;iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.

Serotypes

Cudurka cagaha iyo afkawaxay ku dhacdaa todobada waaweynserotypes: O, A, C, SAT-1, SAT-2, SAT-3, iyo Asia-1.Serotypes-yadani waxay muujinayaan qaar ka mid ah gobollada, iyo O serotype waa kuwa ugu badan.

Nuxurka

 

Reagent

Mugga

96 Tijaabooyin/192 Tijaabooyin

1
Microplate-dahaarka antigen

 

1ea/2e

2
 Xakamaynta taban

 

2.0ml

3
 Xakamaynta togan

 

1.6ml

4
 Tusaalaha dareeraha

 

100ml

5
Xalka dhaqidda (10X xoogga saaray)

 

100ml

6
 Enzyme conjugate

 

11/22 ml

7
 Substrate

 

11/22 ml

8
 Xalka joojinta

 

15ml

9
Xireeyaha saxan koollada

 

2ea/4e

10 mikroplate serum dilution

1ea/2e

11  Tilmaanta

1 pcs

 


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