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Xirmada Cudurka Bursal-ka ee Digaaga ee Ab Elisa

Koodhka badeecada:

Shayga Magaca: Xirmada Cudurka Bursal-ka ee Digaaga ee Ab Elisa

Soo koobid: Xirmada ogaanshaha cudurka caabuqa caabuqa ee digaaga ee caabuqa caabuqa ee dilaaga ah ee ka takhalusaya antibody ee serum digaaga si loo qiimeeyo ka takhalusida unugyada difaaca jirka ee ay soo saartay tallaalka cudurada faafa ee digaaga Heerka iyo ogaanshaha cudurka serological ahaan caawiyay digaagga cudurka.

Bartilmaameedyada Ogaanshaha: Digaagga caabuqa caabuqa bursalka fayraska difaaca jirka

Tusaalaha Tijaabada: Serum

Tilmaam: 1 xirmo = 192 Imtixaan

Kaydinta: Dhammaan reage-yaasha waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

Waqtiga kaydinta: 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.


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Xirmada Cudurka Bursal-ka ee Digaaga ee Ab Elisa

Soo koobid  Ogaanshaha ka-hortagga ka-hortagga caabuqa ee fayraska Fabricius ee serum digaagga
Bartilmaameedyada ogaanshaha Digaagga caabuqa bursalka fayraska difaaca jirka
Tusaale Serum

 

Tirada 1 xirmo = 192 Imtixaan
 

 

Deganaanshaha iyo Kaydinta

1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin

2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.

 

 

 

Xog

Cudurka bursalka ee faafa(IBD), oo sidoo kale loo yaqaano cudurka Gumboro, bursitis infekshanka iyo nephrosis avian, waa cudur aad u faafa oo dhalinyarada ah.digaaggaiyo turkiga uu keeno fayraska caabuqa bursal disease (IBDV), oo lagu gartodifaaca jirkaiyo dhimashada guud ahaan 3 ilaa 6 toddobaad da'da.Cudurka ayaa markii ugu horeysay laga helayGumboro, Delaware1962. Dhaqaale ahaan waxay muhiim u tahay warshadaha digaaga adduunka oo dhan sababtoo ah kororka u nuglaanshaha cudurrada kale iyo faragelinta taban ee waxtarka lehtallaalka.Sanadihii la soo dhaafay, noocyada aadka u daran ee IBDV (vvIBDV), oo sababay dhimashada ba'an ee digaaga, ayaa ka soo baxay Yurub,Laatiin Ameerika,Koonfur-Bari Aasiya, Afrika iyoBariga Dhexe.Caabuqa waxa uu maraa dariiqa saxarada oromo, iyada oo shimbiraha uu saameeyay ay soo saarayaan heerar sare oo fayraska ah qiyaastii 2 toddobaad kadib caabuqa.Cudurku wuxuu si fudud uga qaadaa digaagga cudurka qaba wuxuuna u gudbiyaa digaaga caafimaadka qaba isagoo u mara cunto, biyo, iyo taabashada jirka.

Mabda'a Imtixaanka

Xirmadu waxay isticmaashaa hab tartan ah oo ELISA ah, oo hore loo sii baakeeyay fayraska caabuqa caabuqa ee bursal virus VP2 borotiinka microplate, oo kula tartamo anti-VP2 borotiinka antibody ee serum ee vector wejiga adag iyadoo la isticmaalayo anti-VP2 borotiinka monoclonal antibody.Imtixaanka, antibody-ka monoclonal si loo tijaabiyo iyo borotiin ka-hortagga VP2 ayaa lagu daraa, iyo ka dib marka la tallaalo, haddii muunada ay ku jirto fayraska caabuqa caabuqa ee digaaga ee VP2 borotiinka-gaaska, waxay ku xidhan tahay antigen-ka saxanka dahaarka leh.Taas oo xannibaysa xidhitaanka borotiinka monoclonal anti-VP2 ee antigen-ka, ka dib marka la dhaqo si looga saaro antibody-ka aan xidhnayn iyo qaybaha kale;ka dibna lagu daro anti-mouse-enzyme-ku-calaamadeysan antibody-ka sare si si gaar ah loogu xiro isku-dhafka antigen-antibody ee saxanka ogaanshaha;Isku-xidhka ensaymes-ka ee aan xidhnayn waxa lagu saaraa iyadoo la dhaqay;Substrate-ka TMB ayaa lagu daraa microwell si uu u horumariyo midabka, iyo qiimaha nuugista ee muunada ayaa si xun loola xiriiriyaa waxa ku jira anti-VP2 borotiinka antibody ee ku jira, si loo gaaro ujeedada lagu ogaanayo anti-VP2 antibody ee muunadda.

Nuxurka

 

Reagent

Mugga

96 Tijaabooyin/192 Tijaabooyin

1
Microplate-dahaarka antigen

 

1ea/2e

2
 Xakamaynta taban

 

2.0ml

3
 Xakamaynta togan

 

1.6ml

4
 Tusaalaha dareeraha

 

100ml

5
Xalka dhaqidda (10X xoogga saaray)

 

100ml

6
 Enzyme conjugate

 

11/22 ml

7
 Substrate

 

11/22 ml

8
 Xalka joojinta

 

15ml

9
Xireeyaha saxan koollada

 

2ea/4e

10 mikroplate serum dilution

1ea/2e

11  Tilmaanta

1 pcs

 


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