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Tignoolajiyada ogaanshaha Enzvme ee Enterococcus ee tijaabada biyaha Sawirka
  • Tignoolajiyada ogaanshaha Enzvme ee Enterococcus ee baaritaanka biyaha
  • Tignoolajiyada ogaanshaha Enzvme ee Enterococcus ee baaritaanka biyaha

Tignoolajiyada ogaanshaha Enzvme ee Enterococcus ee baaritaanka biyaha

Koodhka badeecada:

Shayga Magaca:Enzvme technolouv ogaanshaha Enterococcus

Dabeecad: Alaabtani waa qayb jaale ah oo cad ama iftiin ah oo cad

Midab la'aan:ama huruud khafiif ah

pH 7.0 - 7.6

Miisaanka: 2.7 士 0.5g

Kaydinta: Kaydinta 4°C – 8°C, Meel qabow oo qalalan kana ilaali iftiinka

Ansaxinta: 1 sano, eeg baakadaha reagent taariikhda wax soo saarka iyo taariikhda uu dhacayo.

Sayniska: Ku dar muunada biyaha oo ay ku jiraan bakteeriyada Enterococcus, ku dhaqan bakteeriyada bartilmaameedka ah ee ku jirta Mug dhexdhexaad ah 41°C iyo 0.5°C,iyo enzymes-yada gaarka ah ee ay soo saarto bakteeriyada Enterococcus

Mug substrate fluorescent ee ku jira bakeeriga dhexdhexaadka ah si loo soo saaro (3 -D-glucoside ((3 -0 -glucoside) iyo

sheyga fluorescent sifada leh 4-methyl umbelliferon.U fiirso fluorescence ee 366nm UV laambad, ku xisaabi saxanka ogaanshaha tirada, oo weydii miiska MPN si aad u xisaabiso natiijooyinka.

Xidhmada: 100 - xirmo tijaabo ah


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Dabeecad

 Alaabtani waa qaybo huruud ah oo cad ama khafiif ah

Caddayn

 Midab la'aan ama huruud khafiif ah

PH

 7.0 - 7.6

Wsideed

 2.7 士 0.5g

Kaydinta

 at 4°C - 8°C, meel qabow oo qalalan kana ilaali iftiinka

Ansaxnimada

 1 sano, eeg baakadaha reagent taariikhda wax soo saarka iyo taariikhda uu dhacayo.

Sayniska

 Ku dar muunada biyaha oo ay ku jiraan bakteeriyada Enterococcus, dhaqan bakteeriyada bartilmaameedka ah ee ku jirta Mug dhexdhexaad ah 41 ° C iyo 0.5 ° C, iyo enzymes-ka gaarka ah ee ay soo saarto bakteeriyada Enterococcus (β-D-glucoside ((β-D-glucoside) iyo sheyga sifada leh ee fluorescent 4-methyl umbelliferone. U fiirso fluorescence ee 366nm UV laambad, ku tiriso saxanka ogaanshaha tirada, oo weydii miiska MPN si aad u xisaabiso natiijooyinka.

Xidhmada

 100 - xirmo tijaabo ah

Habka isticmaalka Imtixaan tayo leh

Setp1: Qaado muunad 100ml oo biyo ah oo leh 100ml dhalada muunad nadiif ah oo nadiif ah, ku dar reagent, milmi, iyo dhaqan 24h at 41°C 士 0 .5°C

Setp2: Xukunka natiijada

Non fluorescence = Natiijooyin taban oo buluug ah = Natiijooyin togan

Ogaanshaha tirada

1.Isticmaal 100ml dhalada saambalka nadiifka ah si aad u cabbirto muunad 100ml oo biyo ah.

2.Ku dar reagent, milmi, ku shub 51 Quanti Tray ama 97 Quanti-Tray

3. Ku xidh baakadka Quanti-Tray oo leh barnaamij koontaroolaya mashiinka wax lagu xidho, oo ku daadi 41°C 士 0.5°C 24h.

4. Tixraac tijaabada tayada tarjumaada natiijada, oo hubi miiska MPN si aad u tiriyo.

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