Soo koobid | Helitaanka Antibody gaar ah ee Peste Des Petits Ruminants |
Mabda'a | Qalabka baaritaanka PPRV ee ELISA waxaa loo istcmaalaa ogaanshaha Peste des petits ruminants fayraska difaaca jirka ee serumka idaha iyo riyaha. |
Bartilmaameedyada ogaanshaha | PPV antibody |
Tusaale | Serum
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Tirada | 1 xirmo = 192 Imtixaan |
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Ovine rinderpest, oo sidoo kale loo yaqaanpeste des xayawaanka xayawaanka yaryar(PPR), waa cudur la kala qaado oo inta badan saameeyariyahaiyoidaha;si kastaba ha ahaatee, geel iyo duur yaradhijirradasidoo kale waa la saameeyaa.PPR ayaa hadda ku jirtaWaqooyi, Dhexe, GalbeediyoBariga Afrika, ahBariga DhexeiyoKoonfurta Aasiya. Waxaa keenaymorbillivirusee hiddahaMorbillivirus,oo ay aad isugu dhow yihiin, iyo kuwo kale, morbillivirus ugu caansan, jadeecada morbillivirusiyomorbillivirus canine(horey loo yiqiincaninefayraska distemper).Cudurku aad buu u faafaa, wuxuuna yeelan karaa 80-100% heerka dhimashadaba'anxaaladaha ku jira aepizooticdejinta.Fayrasku kuma dhaco dadka.
PPR waxaa sidoo kale loo yaqaan cudurka daacuunka,kata, xanuunka stomatitis-pneumoenteritis, iyo rinderpest geed geedeedka.
Wakaaladaha rasmiga ah sidaFAOiyoOIEisticmaal magaca Faransiiska"peste des xayawaanka xayawaanka yaryar"oo leh dhowr nooc oo kala duwanaansho ah.
Xirmadani waxay isticmaashaa habka ELISA ee tartanka si ay mar hore u dahaadhay antigens PPRV ee ceelasha microplate.Marka la tijaabinayo, ku dar muunada serum la badhxay, ka dib marka la isku duro, haddii ay jirto antibody-ga PPRV, waxay ku dari doontaa antigen-ka hore loo dahaadhay, antibody-ka muunada ku jira ayaa xannibaya isku-darka antibody-ka monoclonal iyo antigen horay loo dahaadhay;iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soo saartaa kicinta Enzyme waxay u dhigantaa saamiga lidka ku ah unugyada unugyada ee muunada.
Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
1 |
| 1ea/2e | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | mikroplate serum dilution | 1ea/2e | |
11 | Tilmaanta | 1 pcs |