Soo koobid | Used si loo ogaado gaar ah antibody ka hortagga Fayraska hargabka Avian (AIV-H5) ee serumka |
Mabda'a | H5 nooc ka mid ah hargabka avian-kaantibodyQalabka Elisa waxaa loo isticmaalaa in lagu ogaado antibody gaar ahAvian Fayraska hargabka (AIV-H5) ee serum, si loola socdo antibody ka dib difaaca AIV-H5iyo ogaanshaha serological ee caabuqa ee Avian.
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Bartilmaameedyada ogaanshaha | H5 subtype antibody hargabka avian |
Tusaale | Serum
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Tirada | 1 xirmo = 192 Imtixaan |
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Hargabka avian, oo si aan rasmi ahayn loo yaqaan ifilada avian ama hargab shimbireedka, waa hargab kala duwan oo uu keeno fayras la qabsadayshimbiraha.
Nooca halista ugu badan leh waa hargabka avian-ka (HPAI).Hargabka shimbiruhu wuxuu la mid yahayifilada doofaarka, ifilada eyda, hargabka faraska iyo
Hargabka bini'aadamka waa cudur ay sababaan noocyada fayrasyada hargabka kuwaas oo la qabsaday qof gaar ah.
Saddexda nooc ee fayraska hargabka (A,BiyoC), fayraska hargabka A waa azoonoticinfekshanka kaydka dabiiciga ah ku dhawaad
Hargabka avian-ka, ujeeddooyinka intooda badan, waxa loola jeedaa fayraska hargabka A.
Xirmadaan waxay isticmaashaa habka ELISA ee xannibaya, AIV-H5 antigen waxaa horay loogu dahaadhay microplate.Marka la tijaabinayo, ku dar muunada serum la badhxay, ka dib marka la isku daro, haddii ay jirto AIV-H5 antibody gaar ah, waxay ku dari doontaa antigen-ka hore loo dahaadhay, iska tuur antibody-ka aan la isku darin iyo qaybaha kale ee dhaqidda;ka dibna ku dar ensaymes ku suntan anti-AIV-H5 antibody monoclonal, antibody muunad ayaa xannibaya isku darka antibody monoclonal iyo antigen hore loo dahaadhay;iska tuur isku xidhka ensaymeska aan la isku darin ee la dhaqo. Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soosaarto Enzyme catalysis waxay u dhigantaa saamiga lidka ku ah unugyada ka hortagga ee muunada..
Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | mikroplate serum dilution | 1ea/2e | |
11 | Tilmaanta | 1 pcs |