Soo koobid | Ogaanshaha qaaxada qaaxada Bovine (BTB) ee ka hortagga unugyada |
Mabda'a | Xirmada Tiibishada Bovine (BTB) ee ka hortagga unugyada Elisa waxaa loo isticmaali karaa in lagu ogaado ka hortagga qaaxada Bovine ee serum ama balaasmaha Bovine. |
Bartilmaameedyada ogaanshaha | Qaaxada Bovine (BTB) kahortaga unugyada |
Tusaale | Serum
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Tirada | 1 xirmo = 192 Imtixaan |
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Mycobacterium bovis waa aayar u koraysa (16 ilaa 20-saacadood wakhtiga jiilka) bakteeriyada aerobic iyo wakiilka qaaxada ee lo'da (oo loo yaqaan TB-da lo'da).Waxay la xiriirtaa Mycobacterium tuberculosis, bakteeriyada keenta tiibishada aadanaha.M. bovis waxa uu ka boodi karaa xayndaabka noocyada oo waxa uu u keeni karaa infekshanka qaaxada u eg ee bini aadamka iyo naasleyda kale.
Tani xirmo isticmaal dadban ELISA habka, daahirin BTB antigen is horay loo dahaadhay on enzyme micro-ceel strips. Markaad tijaabinayso, ku dar milmay serum muunad, ka dib kalluun, if halkaas is BTB gaar ah antibody, it doono isku dar leh ah horay loo dahaadhay antigen, iska tuur ah aan la isku darin antibody iyo kale qaybaha leh dhaqid; markaas ku dar enzyme isku xidhid, iska tuur ah aan la isku darin enzyme isku xidhid leh
dhaqid. Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee kicinta Enzyme waa si toos ah saamiga ka kooban antibody ee muunad.
Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
1 |
| 1ea/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| 15ml | |
9 |
| 2ea/4e | |
10 | mikroplate serum dilution | 1ea/2e | |
11 | Tilmaanta | 1 pcs |