Alaabta
Antigen ELISA Kit
Xirmada ELISA ee caabuqa Cudurka Hydatid
| Soo koobid | loo isticmaalo in lagu ogaado Avian leukosis P27 antigen ee dhiiga avian, saxarada, cloaca, iyo ukunta cad. |
| Mabda'a | Qalabka 'Avian Leukosis' (AL) P27 antigen Elisa kit waxaa loo isticmaalaa in lagu ogaado Avian leukosis P27 antigen ee ku jira dhiiga avian, saxarada, cloaca, iyo ukunta cad.
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| Bartilmaameedyada ogaanshaha | Leukosis Avian (AL) P27 antigen |
| Tusaale | Serum
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| Tirada | 1 xirmo = 192 Imtixaan |
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Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Xog
Avian Leukosis (AL) waa erey wadajir ah oo loogu talagalay cudurrada kala duwan ee la xiriira burooyinka ee digaaga ee uu keeno Fayraska Leukosis Avian (ALV) ee qoyska Retroviridae.Cudurkan waa mid caalami ah oo loo qaybiyo waxaana uu leeyahay heerka caabuqa oo sarreeya.Waxay sababi kartaa dhimasho iyo dildilaac ku dhaca digaagga, hoos u dhigista awoodda wax soo saarka adhiga, waana mid ka mid ah cudurrada ugu waaweyn ee khatarta weyn u geysta horumarinta warshadaha digaaga.Cudurkani waxa uu leeyahay taariikh dheer waxana uu si joogto ah ula kulmaa kiisas cusub, sida koox-hoosaadka fayraska leukemia ee Avian leukemia J (ALV-J), kaas oo la ogaaday dabayaaqadii 1980-aadkii gudaha UK in uu yahay nooc cusub oo fayraska leukemia-ga avian ah, kaas oo keenaya weyn waxyeelada warshadaha broiler
Mabda'a Imtixaanka
Qalabku wuxuu isticmaalaa sandwich ELISA, saafi ah anti-avian leukocyte P27 monoclonal antibody ayaa horay loogu dahaadhay fiilooyinka ceelka-ceelka enzyme. Imtixaanka, antigen-ka ku jira muunada waxay ku xidhan tahay antibody-ka saxanka dahaarka leh, ka dib marka la dhaqo si looga saaro. antigen-ka unbound iyo qaybaha kale, antibody-ka monoclonal enzyme ayaa lagu daraa si gaar ah loogu xiro isku-dhafka antigen-antibody ee saxanka tijaabada.ka dib maydhista, isku xidhka ensaymka ee aan xidhnayn ayaa laga saarayaa, xalka substrate TMB ayaa lagu daraa microplate, calaamada buluuga ah ee kicinta enzyme waxay si toos ah u tahay saamiga antibody-ka ee muunada.Ku dar xal joojinta, falcelinta ka dib, nuugista qiimaha A ee falcelinta si fiican waxaa lagu qiyaasaa mawjadaha dhererka 450 nm.
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
