Qalabka tijaabada ah ee Anaplasma Phagocytofilum Ab | |
Lambarka buugaagta | RC-CF26 |
Soo koobid | Ogaanshaha unugyada difaaca gaarka ah ee Anaplasma10 daqiiqo gudahood |
Mabda'a | Hal tallaabo oo tijaabo ah oo immunochromatographic ah |
Bartilmaameedyada ogaanshaha | Unugyada difaaca jirka ee Anaplasma |
Tusaale | Canine dhiiga oo dhan, serum ama plasma |
Waqtiga akhriska | 5 ~ 10 daqiiqo |
Dareenka | 100.0% vs. IFA |
Gaar ahaan | 100.0% vs. IFA |
Xadka Ogaanshaha | IFA Titer 1/16 |
Tirada | 1 sanduuq (xirmo) = 10 qalab (xirmo shaqsiyeed) |
Nuxurka | Qalabka tijaabada, dhalada kaydka ah, iyo kuwa la tuuri karo |
Digniin | Isticmaal 10 daqiiqo gudahood ka dib furitaankaIsticmaal qadar ku habboon oo muunad ah (0.01 ml oo dhibco ah) Isticmaal 15 ~ 30 daqiiqo ka dib RT haddii ay ku kaydsan yihiin xaalado qabow Tixgeli natiijada imtixaanku inay khaldan yihiin 10 daqiiqo ka dib |
Bakteeriyada Anaplasma phagocytophilum (oo hore u ahaan jirtay Ehrilichia phagocytophila) waxay keeni kartaa infekshan ku dhaca noocyo badan oo xayawaan ah oo uu ku jiro aadanaha.Cudurka ku dhaca ariga guriga waxaa sidoo kale loo yaqaan qandhada shilinta (TBF), waxaana la yaqaanay ugu yaraan 200 oo sano.Bakteeriyada qoyska Anaplasmataceae waa garaam-negative, nonmotile, coccoid ilaa noolaha ellipsoid, oo ku kala duwan cabbirka 0.2 ilaa 2.0um dhexroor.Waxay yihiin aerobes waajib ah, oo aan lahayn dariiqa glycolytic, dhammaantoodna waa dulin ku-meel-gaar ah oo ku-meel-gaar ah.Dhammaan noocyada genus Anaplasma waxay ku nool yihiin vacuoles xuub-ku-saleysan oo ku jira unugyo hematopoietic ah oo aan qaan-gaarin ama qaan-gaar ah ee martida naasleyda.Fagocytofilum wuxuu waxyeeleeyaa neutrophils iyo ereyga granulocytotropic waxaa loola jeedaa neutrophils cudurka qaba.Noolaha dhif ah, ayaa laga helay eosinophils.
Anaplasma phagocytophilum
Calaamadaha caafimaad ee caadiga ah ee anaplasmosis canine waxaa ka mid ah qandho sare, caajisnimo, niyad-jab iyo polyarthritis.Calaamadaha neerfaha (ataxia, suuxdin iyo qoor xanuun) ayaa sidoo kale la arki karaa.Infekshanka Anaplasma phagocytophilum waa dhif dhimasho ilaa ay ku adkaato caabuqyada kale.Khasaaraha tooska ah, xaaladaha curyaaminta iyo khasaaraha wax soo saarka ayaa lagu arkay baraarka.Ilmo soo rididda iyo shahwada daciifka ah ee idaha iyo lo'da waa la duubay.Darnaanta caabuqa waxaa saameeya dhowr arrimood, sida kala duwanaanshaha Anaplasma phagocytophilum ee ku lug leh, cudur-sidaha kale, da'da, heerka difaaca iyo xaaladda qofka martida loo yahay, iyo arrimo sida cimilada iyo maamulka.Waa in la xuso in calaamadaha bukaan-socodka ee bini-aadmiga ay u dhexeeyaan jirro fudud oo hargab-u-eg oo is-xakamaysan, iyo caabuq nafta halis gelinaya.Si kastaba ha ahaatee, inta badan infekshannada bini'aadamka waxay u badan tahay inay keenaan wax yar ama aan lahayn muuqaal caafimaad.
Anaplasma phagocytophilum waxaa lagu kala qaadaa shilinta ixodid.Gudaha Maraykanka faleebooyinka ugu waaweyn waa Ixodes scapularis iyo Ixodes pacificus, halka Ixode ricinus la ogaaday inuu yahay fayraska exophilic ee Yurub.Anaplasma phagocytophilum waxaa si toos ah u gudbiya shilintan vector, mana jirto wax caddaynaya gudbinta transovarial.Inta badan cilmi-baadhisyada ilaa hadda lagu baaray muhiimada ay leeyihiin naasleyda A. phagocytophilum iyo xuubka shilinta ayaa diiradda saaray jiirka laakiin noolahani wuxuu leeyahay naasley ballaaran oo kala duwan, oo uu ku dhaco bisadaha, eyda, idaha, lo'da, iyo fardaha.
Immunofluorescence assay ee aan tooska ahayn waa baaritaanka ugu muhiimsan ee loo isticmaalo in lagu ogaado caabuqa.Shaybaarada serum wejiga degdega ah iyo kuwa qallafsan waa la qiimeyn karaa si loo raadiyo isbeddel afar-laab ah ee titer antibody ilaa Anaplasma phagocytophilum.Intracellular inclusions (morulea) ayaa lagu arkay granulocytes ee Wright ama Gimsa smears dhiiga.Hababka silsiladda polymerase (PCR) ayaa loo isticmaalaa si loo ogaado Anaplasma phagocytophilum DNA.
Ma jiro wax tallaal ah oo la heli karo si looga hortago caabuqa Anaplasma phagocytofilum.Kahortaggu wuxuu ku tiirsan yahay ka fogaanshaha soo-gaadhista shilinta (Ixodes scapularis, Ixodes pacificus, iyo Ixode ricinus) laga bilaabo guga ilaa dayrta, isticmaalka kahortaga ee antiacaricides, iyo isticmaalka prophylactic ee doxycycline ama tetracycline marka la booqanayo Ixodes scapularis, Ixodes pacideckus-ricinus, iyo Ixodes pacificus. gobollada cidhiidhiga ah.