Alaabta
Qalabka Elisa ee tartanka ka hortagga-jirka ee Brucellosis
Qalabka Elisa ee tartanka ka hortagga-jirka ee Brucellosis
| Soo koobid | Helitaanka Kahortagga Gaarka ah ee Foot iyo Cudurka Afka Nooca BRU |
| Mabda'a | Xirmada baaritaanka BRU ee ELISA waxay u isticmaashaa ogaanshaha unugyada difaaca jirka ee Brucellosis ee serumka doofaarka, lo'da, idaha iyo riyaha. |
| Bartilmaameedyada ogaanshaha | Antibody-ka BRU |
| Tusaale | Serum
|
| Tirada | 1 xirmo = 192 Imtixaan |
|
Deganaanshaha iyo Kaydinta | 1) Dhammaan reagents waa in lagu kaydiyaa 2 ~ 8 ℃.Ha bafin 2) Nolosha shelf waa 12 bilood.Isticmaal dhammaan reagen-yaasha ka hor taariikhda uu dhacayo xirmada.
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Mabda'a Imtixaanka
Tusmada ugu hooseysa ee BRU antibodyELISAxirmada tijaabadaisticmaal si ogaanshaha unugyada difaaca jirka ee Brucellosis ee serum ee doofaarka, lo'da, scidhiidhi iyo ari .
Qalabkan isticmaal Habka ELISA ee tartanka hore u-xiraned BRU antigens ee ceelasha microplate.Markaad tijaabinayso, ku dar muunada serum oo la badhxay iyoEnzyme lagu tilmaamay anti-BRU monoclonal antibody, ka dib soo-baxa, haddii ay jiraan leeyihiin BRU antibody, waxay ku dari doontaa antigen-ka hore loo dahaadhay, antibody-ka muunadku waxay xannibaysaa isku darka antibody-ka monoclonal iyo antigen-ka hore loo daboolay;iska tuur isku-xidhka enzyme-ka aan la isku darin ee la dhaqo;Ku dar substrate-ka TMB ee ceelasha-yar-yar, calaamada buluuga ah ee ay soosaarto Enzyme catalysis waxay u dhigantaa saamiga lidka ku ah unugyada ka hortagga ee muunada..
Nuxurka
| Reagent | Mugga 96 Tijaabooyin/192 Tijaabooyin | ||
| 1 |
| 1ea/2e | |
| 2 |
| 2ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4e | |
| 10 | mikroplate serum dilution | 1ea/2e | |
| 11 | Tilmaanta | 1 pcs |
